Vimentin is found in all types of sarcomas and lymphomas. Positive staining for vimentin is seen in most cells of fibrosarcomas, liposarcomas, malignant fibrous histocytomas, angiosarcomas, chondrosarcomas and lymphomas. All melanomas and Schwannomas are strongly vimentin-positive.


a metastatic potential. They are paucicellular, have a myxoid stroma and thick-walled blood vessels. They typically stain for ER, PR, vimentin and desmin.

Description: This monoclonal antibody reacts with the intermediate filament protein vimentin present in cells of mesenchymal origin. Broad interspecies cross-reactivity has been observed with human, rat, and chicken vimentin. a Representative IHC stainings for vimentin and Pax-5 in nt-FL and corresponding s-FL/s-tFL. Stains were performed on consecutive slides. For each stain, the figures shown are from tissue samples Vimentin is present in nasal and lung epithelial cells, and it may also be present in the extracellular space of the lungs and other tissues. Using an anti-vimentin antibody and staining, the title = "CD99, keratin, and vimentin staining of sex cord-stromal tumors, normal ovary, and testis", abstract = "CD99, a marker for MIC-2, reacts with normal Sertoli cells and granulosa cells.

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Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin stainings show proliferation of stellate cells and THP-1 macrophages in the microtissues, suggesting the onset of inflammation process. For full image see PMID 28665955. Flow Cytometry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) Myofibroblasts usually stain for the intermediate filament vimentin which is a general mesenchymal marker and "alpha smooth muscle actin" (human gene = ACTA2) and for palladin, which is a cytoskeletal actin scaffold protein. Mesenchymal and endothelial cells usually stain vimentin positive and thus act as a measure of internal quality control in immunoreactivity. Absence of immunostaining in these areas often indicates that there is significant damage to tissue antigens and loss of structural architecture.

conventional histopathology using nonimmunologic histochemical stains. Summary and explanation Vimentin is a 57 kDa intermediate filament (IF) protein, which form part of the cytoskeleton of vertebrate cells. Among the IFs, vimentin belongs to class III, showing a high degree of specificity for cells of mesenchymal origin.

Denna avbildning avslöjar subdiffraktionsstorleksfunktioner hos de mellanliggande filamenten keratin och vimentin , kritiska i epitelial 

The lymphoepithelium covering the bronchus-associated lymphoid tissue (BALT) of the rabbit lung was studied with monoclonal antibodies against vimentin, using the indirect immunoperoxidase technique. In the lymphoepithelium single cells which had a membranous apical cytoplasm and engulfed intraepithelial lymphocytes were vimentin-immunoreactive.

Odlade celler uttrycker α-SMA och vimentin och express desmin svagt eller inte alls. Odlingsbetingelser är inte bidrar till tillväxten av epitelial, 

Broad interspecies cross-reactivity has been observed with human, rat, and chicken vimentin. OMA1-06001 detects vimentin from mouse, rat, hamster, chicken, goat, canine, monkey, zebrafish, porcine and human tissues and cells. OMA1-06001 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunohistochemistry (frozen) procedures. Vimentin Antibody Staining Protocol for Immunohistochemistry . Description: Vimentin is an intermediate filament that is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Vimentin is useful as part of an antibody panel for differential diagnostic purposes, such as differentiating soft tissue Using immunologic methods, vimentin can be readily demonstrated in frozen sections as well as paraffin sections. However, in formalin-fixed tissue, there may be reduced vimentin staining, and results could be inconsistent.

Vimentin stain

Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig,  av B Sundström · 1990 · Citerat av 2 — cells, the desmin filaments of muscle cells, and the vimentin filaments of display a pluri-epithelial pattern, which besides the "TS 8 backbone" also stain. Hitta stockbilder i HD på vimentin och miljontals andra royaltyfria stockbilder, illustrationer Immunohistochemical stain for vimentin in a seminiferous tubule. a metastatic potential. They are paucicellular, have a myxoid stroma and thick-walled blood vessels.
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Vimentin stain

Immunofluorescence staining of HeLa Cells with antibody to reveal vimentin containing intermediate filaments in green and antibody to LAMP1 to reveal lysosomes in red. Nuclear DNA is seen in blue. Antibodies and image courtesy EnCor Biotechnology Inc. Vimentin is a type III intermediate filament protein that is expressed in Vimentin stains virtually all spindle cell neoplasms—mesenchymal spindle cell neoplasms and sarcomatoid carcinomas included. However, vimentin stains a subset of carcinomas regularly and to a significant degree, and this may be useful in the context of a panel of antibodies to narrow a differential diagnosis.

Among the IFs, vimentin belongs to class III, showing a high degree of specificity for cells of mesenchymal origin. See below.
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Vimentin antibody Mouse Monoclonal from Proteintech validated in Western Blot (WB), IHC staining of human breast cancer using 60330-1-Ig.

(FFPE) human tissues. The clinical interpretation of any staining or its . shows dense staining of olfactory axons with anti-vimentin. The vimentin-like receptor neurons and their axons contain vimentin or a protein of similar  10 May 2016 Immunofluorescence staining with alpaca Nano-Boosters & Nano-Labels is compatible with different fixation protocols and does not require any  9 Jan 2020 Vimentin intermediate filaments localize under the actin cortex in mitosis d the patchy appearance of the vimentin staining in single plane.

The Vimentin antibody stains the intermediate filament network while the Actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites. ARG52345 anti-Nestin antibody [4D11] ICC/IF image.

Cite Vimentin Staining in Mouse 3T3 Fiboplasts Fluorescence staining techniques are frequently used in biological and medical research for diagnosis due to their high specificity and sensitivity. Lamps are widely used, however, these light sources have several drawbacks. Vimentin A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type or One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides Block and slide identifiers should be clearly written and match exactly with the Vimentin is one of the 5 major intermediate filaments found in cells (cytokeratin, vimentin, neurofilament, desmin, and glial fibrillary acid protein [GFAP]). It was classically thought to be limited to mesenchymal cells only, and in the early days of immunohistochemistry, it was thought to be a specific marker for sarcomas and other mesenchymal neoplasms. Immunofluorescent analysis of Vimentin using Vimentin Monoclonal antibody (J144) (Product # MA3-745) shows staining in HeLa cells. Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Vimentin-positiva amnion-mesenkymceller lokaliserades till 1-2 cellskikt under localized, sections were targeted at the black stain left by India Ink needles. ANXA1 KO MIA PaCa-2 visade också reducerat uttryck av vimentin, ett protein Equal volumes of 0.4% trypan blue stain and of the cell suspension were mixed.